APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human P2Y2.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
purinergic receptor P2Y2 , HP2U , P2RU1 , P2U , P2U1 , P2UR , P2Y2 , P2Y2R
Cellular Localization
Cell membrane; Multi-pass membrane protein
Background
The product of this gene belongs to the family of P2 receptors, which is activated by extracellular nucleotides and subdivided into P2X ligand-gated ion channels and P2Y G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor, found on many cell types, is activated by ATP and UTP and is reported to be overexpressed on some cancer cell types. It is involved in many cellular functions, such as proliferation, apoptosis and inflammation. Three transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Mar 2013]
Database
Research Area
DATA IMAGES
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GTX639458 WB Image
Boiled and unboiled HL-60 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with P2Y2 antibody [HL2704] (GTX639458) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX639458 WB Image
Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with P2Y2 antibody [HL2704] (GTX639458) diluted at 1:5000 and competitor's antibody (competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX639458 WB Image
Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (GTX639458) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX639458 WB Image
Boiled and unboiled HL-60 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (GTX639458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX639458 ICC/IF Image
P2Y2 antibody [HL2704] detects P2Y2 protein at cell membrane by immunofluorescent analysis.Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: P2Y2 stained by P2Y2 antibody [HL2704] (GTX639458) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
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REVIEW
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